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3-NT(3-Nitrotyrosine) ELISA Kit

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Product Description

Description Enzyme Immunoassay for the determination of 3-NT.
Method Type Competitive-ELISA
Sensitivity 0.94 ng/mL
Assay Range 1.56~100 ng/mL
Other Name 3-Nitro-L-Tyrosine, Nitrotyrosine
Principle This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 3-NT. During the reaction, 3-NT in the sample or standard competes with a fixed amount of 3-NT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 3-NT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated.Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 3-NT T in the samples is then determined by comparing the OD of the samples to the standard curve.
Intended Use This ELISA kit can be applied to the in vitro quantitative determination of 3-NT concentrations in serum, plasma and other biological fluids.
Specificity This kit recognizes some recombinant and natural 3-NT. No significant cross-reactivity or interference was observed.
Sample Type Serum, plasma and other biological fluids
Kit Components Micro ELISA Plate, 8 x 12 well strips
Reference Standard, 2 vials
Concentrated Biotinylated Detection Ab(100x), 120 µL
Concentrated HRP Conjugate(100x), 120 µL
Reference Standard and Sample Diluent, 20 mL
Biotinylated Detection Ab Diluent, 14 mL
HRP Conjugate Diluent, 14 mL
Concentrated Wash Buffer (25x), 30 mL
Substrate Reagent, 10 mL
Stop Solution, 10 mL
Plate Sealer, 5 pieces
Product Manual
Certificate of Analysis
Other supplies required Microplate reader with 450nm wavelength filter
High-precision transferpettor, EP tubes and disposable pipette tips
37°C Incubator
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer
Storage All reagents in this kit should be stored in accordance with the information on product labels. Unused wells should be returned to the foil pouch with the desiccant pack and resealed until air-tight. The Substrate Reagent should not be kept at -20°C. Avoid exposing reagents to strong light during storage or the incubation process. Caps of reagents should be kept tightly sealed to prevent evaporation and microbial contamination. Erroneous results may occur if reagents are not stored as advised above.

Data Sheet E-EL-0040 (PDF File)

Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. Vials are prone to fall over. Use caution, especially when the lid is off .


Manufactured by : Wuhan Elabscience Biotechnology Co. , Ltd


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