BIOMIMESYS® Adipose Tissue represents a new generation of mimetic hydroscaffold for 3D adipocyte and adipocyte-like cell cultures. Available in a ready-to-use format, it enables the culture of adipocytes and adipocyte-like cells under physiological conditions that are representative of the microenvironment found in adipose tissue. The highly porous nature of the scaffold allows the rapid uptake of nutrients, oxygen, etc. into the cells to create a reproducible study model for all downstream analyses used with 3D adipocytes culture.
Composed of hyaluronic acid (HA) - a major component of the cell’s extracellular matrix (ECM) - biofunctionalized with adipose tissue ECM components (Collagens type I and VI and cell binding domain of Fibronectin), the BIOMIMESYS® Adipose tissue hydroscaffold for 3D adipocyte-like cell cultures is thus an ideal study model because it is;
• Highly porous for the simple extraction of RNA and proteins
• Transparent for direct visualization of cells
• Representative of the adipocytes' microenvironment in terms of cell-cell interactions, matrix composition and cell-matrix interactions
BIOMIMESYS® Adipose is made from HYALURONIC ACID (HA) + Collagen I & VI system, is a major component of the adipocyte extracellular matrix (ECM), where it plays an IMPORTANT ROLE in connective tissue. HA-based scaffolds are formed by HA-grafted RGDS + Collagen I & VI system crosslinking HA with ADH to form reticulated chains. High molecular weight HA is made from Gram+bacteria, which makes it easily reproducible.
> Porosity: 70-170um
> Rheology: Young’s modulus: E= 0.45 ± 0.05kPa
> Swelling ratio = 60 ± 10g/g
Ready to use
BIOMIMESYS® hydroscaffold technology is EASY & READY TO USE. Upon receiving the vacuum sealed 96-well plate open it (under a hood) and add the cells directly on top of the matrix. Changing the culture medium is easy as well. To remove medium, simply draw the medium with a pipette between the matrix and the edge of the well. To refresh the medium, place fresh medium onto the surface of the matrix.
BIOMIMESYS® hydroscaffold has many properties (transparent, porous, and biodegradable) that make it ideal for use with numerous downstream applications. The growing cells are easily retrieved from the matrix by a gentle and rapid procedure, meaning that cells cultured in BIOMIMESYS® may be analyzed using all technologies as shown below.
TRANSPARENT --- Microscopy, Plate Reader (OD, fluorescence, luminescence)
PORIOUS --- PCRs, Western-Blot, ELISA
BIODEGRADABLE --- Flow cytometry
SOLID --- Histology
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