BIOMIMESYS® is a new generation of mimetic hydroscaffold for 3D cell culture. Available in a ready-to-use format, it enables the culture of cells under physiological conditions that are representative of the microenvironment found in whole tissues. The highly porous structure of BIOMIMESYS® allows cells to diffuse into the 3D matrix, where they can fix and begin to develop. The structure and conformation of cells cultured in BIOMIMESYS®, such as cancer cells that spontaneously form spheroids, strongly resemble those formed in vivo. Also, the functionality of the BIOMIMESYS® matrix is demonstrated by the expression of genes and proteins at levels that are again more similar to those found in vivo. Cells cultured in BIOMIMESYS® give results that are more physiologically relevant than those grown in 2D culture and are closer to those obtained in vivo.
Composed of Hyaluronic acid (HA) - a major component of the cell’s extracellular matrix (ECM) - the BIOMIMESYS®Oncology hydroscaffold for 3D hepatocyte cultures is an ideal study model because it is;
•Highly porous for the simple extraction of RNA and proteins
•Biodegradable for easy cell retrieval
•Transparent for direct visualization of cells
•Representative of the cell micro-environment in terms of cell-cell interactions, cell-matrix interactions and the expression of proteins and genes
BIOMIMESYS® Oncology is made from HYALURONIC ACID (HA), a glycosaminoglycan.
Hyaluronic acid is a major component of the extracellular matrix (ECM), and it plays an IMPORTANT ROLE in connective tissue.
> Porosity: 150-200um
> Rheology: Young’s modulus: E= 0.5 ± 0.15kPa
> Swelling ratio = 75 ± 10g/g
Ready to use
BIOMIMESYS® hydroscaffold technology is EASY & READY TO USE. Upon receiving the vacuum sealed 96-well plate open it (under a hood) and add the cells directly on top of the matrix. Changing the culture medium is easy as well. To remove medium, simply draw the medium with a pipette between the matrix and the edge of the well. To refresh the medium, place fresh medium onto the surface of the matrix.
BIOMIMESYS® hydroscaffold has many properties (transparent, porous, and biodegradable) that make it ideal for use with numerous downstream applications. The growing cells are easily retrieved from the matrix by a gentle and rapid procedure, meaning that cells cultured in BIOMIMESYS® may be analyzed using all technologies as shown below.
TRANSPARENT --- Microscopy, Plate Reader (OD, fluorescence, luminescence)
PORIOUS --- PCRs, Western-Blot, ELISA
BIODEGRADABLE --- Flow cytometry
SOLID --- Histology
Successfully Tested Cells:
Cancer Cells Lines & Primary Tumors
Human brain metastasis: SA87
Human breast adenocarcinoma : MCF-7
Human breast carcinoma : CAL-51
Human cervix adenocarcinoma : HeLa
Human colorectal adenocarcinoma : DLD-1, HT29, Caco-2
Human glioblastoma : CB109 / CB74 / CB191
Human liver hepatocellular carcinoma : HepG2
Human liver hepatoma : PLC / PRF-5
Human lung carcinoma : NCI-H460
Human osteosarcoma : SaOs
Human ovarian carcinoma : IGROV-1
Human pancreas carcinoma : PANC-1
Human prostate cancer : PC3
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