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iMatrix-511 SILK (Transgenic Silkworm Cocoon)(Size 175ug - Sample Size) *** One Time Purchase Only ***

Price:
$80.00
SKU:
N-892022
Shipping:
Free Shipping

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Product Description

iMatrix-511 SILK

Laminin 511 is known to bind to Intergin α6β1 of the cell surface.

iMatrix-511 SILK is recombinant human Laminin 511-E8 fragments expressed by transgenic silkworm cocoon.

Product Size : 175ug  (175ug x 1 pcs.)

<Application>

iMatrix-511 SILK can be used as cell culture matrix for various cell types including ES/iPS cells.

<Contents>

Liquid Solution (175ug/vial @0.5mg/mL Solvent: PBS(-)) 

**Specification **
Format: Solution (175ug/vial @0.5mg/mL)
Storage temperature: 2-15°C

<Activity>

The dissociation constant of the binding activity with integrin α6β1 is under 10nM.

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<Procedure>

1. Dilute the solution with sterile PBS (-). Coat dishes with 0.5ug/cm². Add 2mL of diluted iMatrix-511 solution to the well.

* The optimum coating concentration depends on cell lines from 0.1 to 1.5ug/cm². (For example, when you use 6-well plate (9.6 cm²/well), add 9.6ul iMatrix-511 (4.8ug) in 1.99ml of PBS(-).)

2. Incubate for 1 hour at 37°C, 3 hours at room temperature, or over night at 4°C.

3. Remove remaining fluid from the coated surface. No rinse is needed.

4. Immediately plate the cells at desired density.

*** Don't allow the plate to dry.

**** Briefly spin down all liquid in the tube before use.

***** Avoid repeated freeze-thaw cycles.

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****When culturing ES/iPS cell, Feeder Free and Single cell subculture can be done using iMatrix-511.*****

*License to manufacture and sell this product has been obtained from Osaka University & Kyoto University.*

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[Reference Literature] 
1. Ido H et al. J. Biol. Chem. 282(15):11144-54, 2007

2. Taniguchi Y et al. J Biol. Chem. 284 (12): 7820-31, 2009

3. Miyazaki T et al. Nat. Commun. 3 1236, 2012

4. Nakagawa M et al. Sci. Rep. 4 3594, 2014

5. Doi D et al. Stem Cell Reports. 2(3):337-50,2014

6. Takashima Y et al. Cell. 158 (6): 1254-69,2014

7. Fukuta M et al. PLoS One. 9(12):e112291,2014

8. Burridge PW et al. Nat. Methods. 11 855-60, 2014

9. Okumura N et al. Invest Ophthalmol Vis Sci. 56(5), 2933-42, 2015

10. Sasaki K et al. Nature 531,367-80, 2016

11. Matsuno Ket al.Differentiation 2016.04.002

12. Nishimura Ket al. Stem Cell Reports 6 (4): 511-524, 2016

13. Takayama K et al. Biochemical and Biophysical Research Communications 474 (1): 91-96, 2016

Questions about this product? Ready to place an order? Email us at orders@iwai-chem.com or give us a call: (650) 486-1541

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