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iMatrix-511 (Size 175ug - Sample Size) *** One Time Purchase Only ***

Price:
$80.00
SKU:
N-892013
Shipping:
Free Shipping
Maximum Purchase:
1 unit(s)

Click the button below to add the iMatrix-511 (Size 175ug - Sample Size) *** One Time Purchase Only *** to your wish list.

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Product Description

iMatrix-511

Laminin 511 is known to bind to Intergin α6β1 of the cell surface.

iMatrix-511 is highly purified human Laminin 511-E8 fragments recombinantly expressed by CHO-S cell.

Product Size : 175ug  (175ug x 1 pcs.)

<Application>

iMatrix-511 can be used as cell culture matrix for various cell types including ES/iPS cells.

<Contents>

Liquid Solution (175ug/vial @0.5mg/mL Solvent: PBS(-)) .

**Specification **
Format: Solution (175ug/vial @0.5mg/mL)
Storage temperature: 2-15°C
NaCl concentration: 137mM

<Activity>

The dissociation constant of the binding activity with integrin α6β1 is under 10nM.

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<Procedure>

1. Dilute the solution with PBS (-). Coat dishes with 0.5ug/cm².

* The optimum coating concentration depends on cell lines from 0.1 to 1.5ug/cm².

** For example, when you use 6-well plate (9.6 cm²/well), add 9.6ul iMatrix-511 (4.8ug) in 1.99ml of PBS(-). Add 2mL of diluted iMatrix-511 solution to the well.

2. Incubate for 1 hour at 37C, 3 hours at Room temperature, or over night at 4C.

3. Remove excess fluid from the coated surface. No rinse is needed.

4. Immediately plate the cells at desired density.

*** Don't allow the plate to dry.

**** Briefly spin down all liquid in the tube before use.

***** Avoid repeated freeze-thaw cycles.

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****When culturing ES/iPS cell, Feeder Free and Single cell subculture can be done using iMatrix-511.*****

*License to manufacture and sell this product has been obtained from Osaka University & Kyoto University.*

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[Reference Literature]
1. Miner, JH and Yurchenco, PD Laminin functions in tissue morphogenesis. Annu. Rev. Cell Dev. Biol. 20, 255-284 (2004).

2. Ido H, Harada K, Futaki S, Hayashi Y, Nishiuchi R, Natsuka Y, Li S, Wada Y, Combs AC, Ervasti JM and Sekiguchi K. Molecular dissection of the alpha-dystroglycan- and integrin-binding sites within the globular domain of human laminin-10. J. Biol. Chem. 279, 10946-10954 (2004).

3. Ido H, Nakamura A, Kobayashi R, Ito S, Li S, Futaki S and Sekiguchi K. The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin gamma chains in integrin binding by laminins. J. Biol. Chem. 282, 11144-11154 (2007).

4. Taniguchi Y, Ido H, Sanzen N, Hayashi M, Sato-Nishiuchi R, Futaki S and Sekiguchi K. The C-terminal region of laminin beta chains modulates the integrin binding affinities of laminins. J. Biol. Chem. 284, 7820-7831 (2009).

5. Miyazaki T, Futaki S, Suemori H, Taniguchi Y, Yamada M, Kawasaki M, Hayashi M, Kumagai H, Nakatsuji N, Sekiguchi K and Kawase E. Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nat. Commun. 3, 1236 (2012).

6. Nakagawa M, Taniguchi Y, Senda S, Takizawa N, Ichisaka T, Asano K, Morizane A, Doi D, Takahashi J, Nishizawa M, Yoshida Y, Toyoda T, Osafune K, Sekiguchi K, Yamanaka S. A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells. SCIENTIFIC REPORTS | 4 : 3594 | DOI: 10.1038/srep03594 2014 Jan. 8

7. Yasuhiro Takashima, Ge Guo, Remco Loos, Jennifer Nichols, Gabriella Ficz, Felix Krueger, David Oxley, Fatima Santos, James Clarke, William Mansfield, Wolf Reik, Paul Bertone, Austin Smith  Resetting Transcription Factor Control Circuitry toward Ground-State Pluripotency in Human.  Cell Volume 158, Issue 6, p1254–1269, 11 September 2014 DOI: 10.1016/j.cell.2014.08.029

8. Doi D et al. Isolation of Human Induced Pluripotent Stem Cell-Derived Dopaminergic Progenitors by Cell Sorting for Successful Transplantation.
Stem Cell Reports. 2 (3): 337-50, 2014

9. Takashima Y et al. Resetting Transcription Factor Control Circuitry toward Ground-State
Pluripotency in Human. Cell. 158 (6): 1254-69, 2014

10. Fukuta M et al. Derivation of mesenchymal stromal cells from pluripotent stem cells through a neural crest lineage using small molecule compounds with defined media.
PLos One. 9 (12) : e112291, 2014

11. Hayashi et al. Structure-based discovery of NANOG variant with enhanced properties to promote self-renewal and reprogramming of pluripotent stem cells.  PNAS vol. 112 (15): 4666-4671, April 14, 2015

FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Manufactured by : Nippi, Inc.

Questions about this product? Ready to place an order? Email us at orders@iwai-chem.com or give us a call: (650) 486-1541

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