BioMIMESYS® is an organ-specific Extracellular Matrix (ECM) formed by crosslinking reaction of hydrosoluble modified hyaluronic acid (HA) and other ECM components (collagens, fibronectin, etc.) with ADH (Adipic acid dihydrazide), to create a "Hydroscaffold" 3D cell culture system.
BIOMIMESYS® Brain represents a new generation of mimetic hydroscaffold for 3D neuronal cell cultures. Available in a ready-to-use format, it enables the long-term culture of neuronal cells by mimicking the components of brain ECM and the physiological conditions that are representative of the microenvironment found in the brain. The highly porous nature of the scaffold allows the rapid uptake of nutrients, oxygen, etc. into the cells to create a reproducible study model for all downstream analyses used with 3D neuronal cells.
Components & Physicochemical Features:
- Components: Collagen IV, cell binding domain of fibronectin (RGDS), cationic bioplolymer, hyaluronic acid.
- High Porosity: 150 ± 30µm
- Young’s modulus: 0.1 kPa
- 80% empty volume provides enough space for gas, media and nutrients flow circulation of fragile neuronal cell with cell-cell and cell-matrix interactions.
ECM has an impact on neuronal cell growth and important in in vitro studies.
Primary rat neuron from cortices (embrionic-day 18) were cultured in brain ECM. The results showed that brain ECM and matricellular proteins significantly improved cell viability, neural network formation and decreased glutamate release. Fetal brain ECM induced superior neuronal network formation, calcium signaling and spontaneous spiking activity compared to adult brain ECM (Sood et al. 2016).