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BioMIMESYS® is an organ-specific Extracellular Matrix (ECM) formed by the crosslinking reaction of hydrosoluble modified hyaluronic acid (HA) and other ECM components (collagens, fibronectin, etc.) with ADH (Adipic acid dihydrazide), to create a "Hydroscaffold" 3D cell culture system.
BIOMIMESYS® Liver represents a new generation of mimetic hydroscaffolding for 3D hepatocyte-like cell cultures. Available in a ready-to-use format, it enables the culture of hepatocytes and hepatocyte-like cells under physiological conditions that are representative of the microenvironment found in liver tissue. The highly porous nature of the hydroscaffold allows the rapid uptake of nutrients, oxygen, etc. into the cells to create a reproducible study model for all downstream analyses used with 3D hepatocytes-like cells culture (metabolism, toxicity, etc.).
BIOMIMESYS® Liver is easy & ready-to-use. Upon receiving the vacuum sealed 96-well plate open it (under a hood) and add the cells directly on top of the matrix. Changing the culture medium is easy as well. To remove medium, simply draw the medium with a pipette between the matrix and the edge of the well. To refresh the medium, place fresh medium onto the surface of the matrix.
Composition of the Scaffold
• Hyaluronic acid (HA) - a major component of the cell’s extracellular matrix (ECM)
• Biofunctionalized with liver ECM components - collagen type I and IV, glycoproteins and glycosaminoglycans (GAG).
• Adipic acid dihydrazide (ADH) as crosslinkers
• Porosity: 117±23μm
• Rheology: Young’s modulus: 0.6kPa
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