Product Description
| Description | Enzyme Immunoassay for in vitro quantitative determination of TGF-β1 concentrations in serum, plasma and other biological fluids. |
| Method Type | Sandwich-Ab |
| Sensitivity | 0.10 ng/mL |
| Reactivity | Universal |
| Assay Range | 0.16—10 ng/mL |
| Assay time | 3.5h |
| Reactivity | Universal |
| Detection Method | Colormetric |
| Sample Volume Per Well | 100uL |
| Principle | This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TGF-β1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TGF-β1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro-plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TGF-β1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of TGF-β1. You can calculate the concentration of TGF-β1 in the samples by comparing the OD of the samples to the standard curve. |
| Intended Use | This ELISA kit can be applied to the in vitro quantitative determination of Human MCP-1 concentrations in serum, plasma and other biological fluids. |
| Specificity | This kit recognizes TGF-β1 in samples. No significant cross-reactivity or interference between TGF-β1 and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
| Kit Components | Micro ELISA Plate, 8 x 12 well strips Reference Standard, 2 vials Concentrated Biotinylated Detection Ab(100x), 120 µL Concentrated HRP Conjugate(100x), 120 µL Reference Standard and Sample Diluent, 20 mL Biotinylated Detection Ab Diluent, 14 mL HRP Conjugate Diluent, 14 mL Concentrated Wash Buffer (25x), 30 mL Substrate Reagent, 10 mL Stop Solution, 10 mL Plate Sealer, 5 pieces Product Manual Certificate of Analysis |
| Other supplies required | Microplate reader with 450nm wavelength filter High-precision transferpettor, EP tubes and disposable pipette tips 37°C Incubator Deionized or distilled water Absorbent paper Loading slot for Wash Buffer |
| Storage | All reagents in this kit should be stored in accordance with the information on product labels. Unused wells should be returned to the foil pouch with the desiccant pack and resealed until air-tight. The Substrate Reagent should not be kept at -20°C. Avoid exposing reagents to strong light during storage or the incubation process. Caps of reagents should be kept tightly sealed to prevent evaporation and microbial contamination. Erroneous results may occur if reagents are not stored as advised above. |
Data Sheet E-EL-0162 (PDF File)
Citations
- Effects And Mechanisms Of Geniposide On Rats With Adjuvant Arthritis.
IF: 2.956
Journal: International Immunopharmacology(2014)
DOI: 10.1016/j.intimp.2014.02.021
PMID: 24583144
Products: E-EL-R0014 E-EL-R0015 E-EL-R0559 E-EL-0162
Sample type: Cell lysate
Reactivity: Rat - Galectin-9 Ameliorates Respiratory Syncytial Virus-Induced Pulmonary Immunopathology Through Regulating The Balance Between Th17 And Regulatory T Cells.
IF: 2.628
Journal: Virus Research(2014)
DOI: 10.1016/j.virusres.2014.10.011
PMID: 25451068
Products: E-EL-R0016 E-EL-R0566 E-EL-0162 E-EL-R0009
Sample type: Tissue homogenate
Reactivity: Rat
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Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. Vials are prone to fall over. Use caution, especially when the lid is off .
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Manufactured by : Wuhan Elabscience Biotechnology Co. , Ltd
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