|Description||Enzyme Immunoassay for the determination of Human Methylase .|
|Assay Range||3.13~200 ng/mL|
|Principle||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Methylase . Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human Methylase and Avidin- Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human Methylase , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme- substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Methylase . The concentration of Human Methylase in samples can be calculated by comparing the OD of the samples with the standard curve.|
|Intended Use||This ELISA kit can be applied to the in vitro quantitative determination of Human Methylase concentrations in serum, plasma and other biological fluids.|
|Specificity||This kit recognizes some recombinant and natural Human Methylase . No significant cross-reactivity or interference was observed.|
|Sample Type||Serum, plasma and other biological fluids|
|Kit Components||Micro ELISA Plate, 8 x 12 well strips Reference Standard, 2 vials Reference Standard and Sample Diluent, 20 mL Concentrated Biotinylated Detection Ab, 120 µL Biotinylated Detection Ab Diluent, 10 mL Concentrated HRP Conjugate, 120 µL HRP Conjugate Diluent, 10 mL Concentrated Wash Buffer (25x), 30 mL Substrate Reagent, 10 mL Stop Solution, 10 mL Plate Sealer, 5 pieces Manual Desiccant, 1 pack Certificate of Analysis|
|Other supplies required||Microplate reader with 450nm wavelength filter High-precision transferpettor, EP tubes and disposable pipette tips 37°C Incubator Deionized or distilled water Absorbent paper Loading slot for Wash Buffer|
|Storage||All the reagents in kit should be stored according to labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20? (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.|
Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. Vials are prone to fall over. Use caution, especially when the lid is off .
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Manufactured by : Wuhan Elabscience Biotechnology Co. , Ltd
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