Product Description
HemEX™ For the expansion of mouse hematopoietic stem cells.
HemEx® - Type9A is a basal medium specifically designed for the expansion of mouse hematopoietic stem cells (HSCs) isolated from bone marrow. Contaminants derived from albumin can trigger the differentiation of HSCs. By substituting albumin with polyvinyl alcohol (PVA), HemEx® - Type9A can maintain HSCs in an undifferentiated state for over one month during expansion culture.
Key Features:
- Serum-free formulation.
- Can maintain HSCs in an undifferentiated state for over one month of expansion culture.
- Verified proliferation of mouse HSCs and hematopoietic stem and progenitor cells (HSPCs).
- Developed under the supervision of Prof. Hiromitsu Nakauchi (Stanford University) and Prof. Satoshi Yamazaki (Tokyo University), based on the Wilkinson et al. 2019 publication.
HemEX-Type9A
Mouse HSC expansion basal medium
100mL
*Requires the addition of cytokines*
The HemEx contains the following.
F12 medium
1mM glutamine
10 mM HEPES
1 mg/mL PVA
X1 ITSX
*Penicillin and streptomycin are not in HemEx but you may add it in if you like.
*Cytokines (SCF and TPO) are not included as well.
Publication:
Wilkinson, A. C. et al. Long-term ex vivo hematopoietic-stem-cell expansion allows nonconditioned transplantation. Nature 571, 117–121 (2019) doi: 10.1038/s41586-019-1244-x.
For more information about this product please contact us.
For Research Use Only
Manufactured by: Cell Science & Technology Institute Inc. (CSTI)
